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Online Ris2Bib – Convert Ris references to BibTex Format
Voltage Calculator
Simple Molarity Calculator
Molarity Calculator from molar mass
Reverse Complement
Taskbar separator
I was looking for a way to organise my Windows taskbar to make it easier for opening private vs work shortcuts. After a bit of searching, I found this perfect GitHub project: https://github.com/DrummerSi/TaskSeparator11 It does require Windows desktop runtime (6.0.24), … Read More »
Cygwin ‘Bash Prompt Here’ for Windows 11
It is always frustrating to have to navigate via bash to the working folder, so after a bit of Googling (thanks StackOverflow), this is how to set up Cygwin bash as a right-click option. Working in Windows 11. The first … Read More »
TAE (Tris/Acetic acid/EDTA) buffer
TAE buffer is used primarily for DNA gel electrophoresis 50x Stock Solution Component Mw(g.mol-1) 1 L Tris base (C4H11NO3) 121.14 242 g Glacial acetic acid (17.5 M) 60.05 57.1 mL 0.5 M EDTA (pH 8.0) – 100 mL dissolve 242 … Read More »
Antibiotic Stock Solutions for Bio Laboratory Use
Common antibiotic stock solutions for laboratory use.
Luria Broth (LB) Medium and LB Agar for E. coli growth
This protocol outlines preparation preparation of Luria Broth (LB) and LB Agar for plates.
Preparation of Competent Agrobacterium Cells for transformation
This protocol describes a method for preparing Agrobacterium for freeze-thaw transformation technique.
Freeze-Thaw Transformation of Agrobacterium tumafaciens
This protocol describes a method for transforming Agrobacterium with plasmid DNA using a freeze-thaw technique. Although the transformation efficiency for Agrobacterium is lower than that for E. coli, it is possible to obtain adequate numbers of transformants with this technique.
Common Stock Solutions for Bio Laboratory Use
A collection (from various sources) of some common stock solutions for use in bio labs.
Preparation of competent E. coli for heat-shock transformation
Standard protocol for preparation of competent cells for transformation with heat-shock method.
Heat-Shock Transformation of E. Coli
Standard protocol for Heat-Shock Transformation of E. Coli.