Tools

Online Tools

Voltage Calculator.
Simple Molarity Calculator.
Molarity Calculator from molar mass.
Reverse Complement.

Other Online Tools

Online Ris2Bib – Convert Ris references to BibTex Format

Voltage Calculator

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Simple Molarity Calculator

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Molarity Calculator from molar mass

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Reverse Complement

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Taskbar separator

I was looking for a way to organise my Windows taskbar to make it easier for opening private vs work shortcuts. After a bit of searching, I found this perfect GitHub project: https://github.com/DrummerSi/TaskSeparator11 It does require Windows desktop runtime (6.0.24), … Read More »

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Cygwin ‘Bash Prompt Here’ for Windows 11

It is always frustrating to have to navigate via bash to the working folder, so after a bit of Googling (thanks StackOverflow), this is how to set up Cygwin bash as a right-click option. Working in Windows 11. The first … Read More »

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TAE (Tris/Acetic acid/EDTA) buffer

TAE buffer is used primarily for DNA gel electrophoresis 50x Stock Solution Component Mw(g.mol-1) 1 L Tris base (C4H11NO3) 121.14 242 g Glacial acetic acid (17.5 M) 60.05 57.1 mL 0.5 M EDTA (pH 8.0) – 100 mL dissolve 242 … Read More »

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Antibiotic Stock Solutions for Bio Laboratory Use

Common antibiotic stock solutions for laboratory use.

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Luria Broth (LB) Medium and LB Agar for E. coli growth

This protocol outlines preparation preparation of Luria Broth (LB) and LB Agar for plates.

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Preparation of Competent Agrobacterium Cells for transformation

This protocol describes a method for preparing Agrobacterium for freeze-thaw transformation technique.

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Freeze-Thaw Transformation of Agrobacterium tumafaciens

This protocol describes a method for transforming Agrobacterium with plasmid DNA using a freeze-thaw technique. Although the transformation efficiency for Agrobacterium is lower than that for E. coli, it is possible to obtain adequate numbers of transformants with this technique.

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Common Stock Solutions for Bio Laboratory Use

A collection (from various sources) of some common stock solutions for use in bio labs.

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Preparation of competent E. coli for heat-shock transformation

Standard protocol for preparation of competent cells for transformation with heat-shock method.

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Heat-Shock Transformation of E. Coli

Standard protocol for Heat-Shock Transformation of E. Coli.

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