TAE buffer is used primarily for DNA gel electrophoresis
50x Stock Solution
| Component | Mw (g.mol-1) | 1 L |
| Tris base (C4H11NO3) | 121.14 | 242 g |
| Glacial acetic acid (17.5 M) | 60.05 | 57.1 mL |
| 0.5 M EDTA (pH 8.0) | – | 100 mL |
- dissolve 242 g Tris base in 700mL of dH₂O.
- add 100 mL 0.5 M EDTA.
- add 57.1 mL Glacial acetic acid.
- adjust pH with 1M NaOH/HCL if required.
- add dH₂O to final volume.
- Autoclave if required, but not usually done.
- Stable at room temperature for a long time. Dispose of if precipitates form.
1x Working Solution
- add 1 volume of 50x TAE to 49 volumes of dH₂O.
- I.e. add 40 mL to 1960 mL of dH₂O for 2L of 1x TAE.
- Generally, 1x TAE can be used for a couple of gel runs as long as there is no significant evaporation.
