Antibiotic Stock Solutions for Bio Laboratory Use
Common antibiotic stock solutions for laboratory use.
Biotechnology and Botany
Experimental protocols
Common antibiotic stock solutions for laboratory use.
This protocol outlines preparation preparation of Luria Broth (LB) and LB Agar for plates.
This protocol describes a method for preparing Agrobacterium for freeze-thaw transformation technique.
This protocol describes a method for transforming Agrobacterium with plasmid DNA using a freeze-thaw technique. Although the transformation efficiency for Agrobacterium is lower than that for E. coli, it is possible to obtain adequate numbers of transformants with this technique.
A collection (from various sources) of some common stock solutions for use in bio labs.
Standard protocol for preparation of competent cells for transformation with heat-shock method.
Standard protocol for Heat-Shock Transformation of E. Coli.
While not “best-practice”, this protocol works well if you don’t need well-purified plasmids or if you don’t have a commercial plasmid mini-prep kit on hand.
A common technique to preserve E. Coli containing specific plasmids (and other bacterial) longer term.
Super Optimal Broth (SOB) is a common bacterial growth medium used for Escherichia coli. Especially useful for assisting in recovery following transformation.