TE (Tris/EDTA) Buffer

21 Mar

TE buffer is used primary for resuspending nucleic acids. Various pHs for different end uses.

Component500 mL1 L
1 M Tris-HCL (pH 7.4, 7.6, or 8.0)5.0 mL10.0 mL
0.5 M EDTA (pH 8.0)1.0 mL2 mL
  • adjust to desired pH with 1M HCL.
  • add dH₂O to final volume.
  • autoclave.

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